Phosphorylation of the N-terminal domain ofXenopusTATA-box binding protein by DNA-dependent protein kinase depends on the C-terminal core domain
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Phosphorylation of p68 RNA helicase regulates RNA binding by the C-terminal domain of the protein.
We previously reported ATPase, RNA unwinding, and RNA-binding activities of recombinant p68 RNA helicase that was expressed in Escherichia coli. Huang et al. The recombinant protein bound both single-stranded (ss) and double-stranded (ds) RNAs. To further characterize the substrate RNA binding by p68 RNA helicase, we expressed and purified the recombinant N-terminal and C-terminal domains of th...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1996
ISSN: 0014-5793
DOI: 10.1016/0014-5793(96)00420-6